Estefani Tavares Jansen, Elder Pacheco da Cruz, Laura Martins Fonseca, Marjana Rad\u00fcnz, Taiane Mota Camargo, Alvaro Renato Guerra Dias, Elessandra da Rosa Zavareze<\/p>\n
The escalating prevalence of lifestyle- and aged-related conditions, including diabetes, chronic inflammation, and cardiovascular disorders, underscores the urgent need for natural therapies. Such alternatives should offer reduced side effects compared to conventional pharmaceuticals while playing a proactive role in disease prevention. Red wine production generates grape pomace, a by-product rich in valuable yet unstable anthocyanins. Encapsulation by electrospinning can protect these compounds. This study aimed to encapsulate grape pomace extract (5%, 10%, and 15%,\u00a0w\/w<\/i>) into electrospun ultrafine zein fibers and to evaluate the\u00a0in vitro<\/i>\u00a0biological activities, including antioxidant, antihyperglycemic, and anti-inflammatory properties.<\/p>\n<\/section>\n Encapsulated grape pomace extract demonstrated antioxidant activity, showing the ability to capture hydroxyl and nitric oxide free radicals. It exhibited an antihyperglycemic effect by inhibiting carbohydrate-digesting enzymes, \u03b1-amylase, and \u03b1-glucosidase. Anti-inflammatory activity was observed through the inhibition of thermal protein denaturation. The evaluated activities were concentration-dependent, with fibers containing 15% (w\/w<\/i>) extract showing the highest\u00a0in vitro<\/i>\u00a0bioactivity.<\/p>\n<\/section>\n Our findings suggest that grape pomace extract encapsulated in ultrafine zein fibers is a promising new formulation for natural medicine. This potential extends to being integrated into functional foods, offering a dual application for health promotion and potential uses in food and pharmaceutical products. \u00a9 2026 The Author(s).\u00a0Journal of the Science of Food and Agriculture<\/i>\u00a0published by John Wiley & Sons Ltd on behalf of Society of Chemical Industry.<\/p>\nRESULTS<\/h3>\n
CONCLUSION<\/h3>\n